8k5p

Electron Microscopy
2.8Å resolution

Cryo-EM structure of yeast Rat1-bound Pol II pre-termination transcription complex 2 (Pol II Rat1-PTTC2)

Released:
Related structures: EMD-36908

Function and Biology Details

Reaction catalysed:
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
hetero octadecamer (preferred)
PDBe Complex ID:
PDB-CPX-270579 (preferred)
Entry contents:
15 distinct polypeptide molecules
2 distinct DNA molecules
1 distinct RNA molecule
Macromolecules (18 distinct):
DNA-directed RNA polymerase II subunit RPB1 Chain: A
DNA-directed RNA polymerase II subunit RPB2 Chain: B
Molecule details ›
Chain: B
Length: 1259 amino acids
Theoretical weight: 143.34 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P08518 (Residues: 1-1224; Coverage: 100%)
Gene names: RPB150, RPB2, RPO22, YOR151C
Sequence domains:
DNA-directed RNA polymerase II subunit RPB3 Chain: C
DNA-directed RNA polymerases I, II, and III subunit RPABC1 Chain: E
DNA-directed RNA polymerases I, II, and III subunit RPABC2 Chain: F
DNA-directed RNA polymerases I, II, and III subunit RPABC3 Chain: H
DNA-directed RNA polymerase II subunit RPB9 Chain: I
DNA-directed RNA polymerases I, II, and III subunit RPABC5 Chain: J
DNA-directed RNA polymerase II subunit RPB11 Chain: K
DNA-directed RNA polymerases I, II, and III subunit RPABC4 Chain: L
Transcription elongation factor SPT5 Chain: W
DNA-directed RNA polymerase II subunit RPB4 Chain: D
DNA-directed RNA polymerase II subunit RPB7 Chain: G
5'-3' exoribonuclease 2 Chain: M
Decapping nuclease RAI1 Chain: O
DNA (38-MER) Chain: N
DNA (38-MER) Chain: T
RNA Chain: P

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.8Å
Relevant EMDB volumes: EMD-36908
Expression system: Escherichia coli BL21(DE3)