› 8k5s

X-ray diffraction
2.65Å resolution

The structure of EntE with 3-(prop-2-yn-1-yloxy)benzoic acid sulfamoyl adenosine

Released:
DOI: 10.2210/pdb8k5s/pdb
PDB entry 8k5s coloured by chain, front view.
PDB entry 8k5s coloured by chain, side view.
PDB entry 8k5s coloured by chain, top view.
Source organism: Escherichia coli
Entry authors: Miyanaga A, Ishikawa F

Function and Biology Details

Reactions catalysed: 
(1a) ATP + 2,3-dihydroxybenzoate = diphosphate + (2,3-dihydroxybenzoyl)adenylate
6 ATP + 3 2,3-dihydroxybenzoate + 3 L-serine = enterobactin + 6 AMP + 6 diphosphate
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-273134 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Enterobactin synthase component E Chains: A, B
Molecule details ›
Chains: A, B
Length: 556 amino acids
Theoretical weight: 61.33 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P10378 (Residues: 1-536; Coverage: 100%)
Gene names: JW0586, b0594, entE
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand VPT 2 x VPT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Unit cell:
a: 124.041Å b: 57.649Å c: 171.427Å
α: 90° β: 96.64° γ: 90°
R-values:
R R work R free
0.189 0.188 0.219
Expression system: Escherichia coli BL21(DE3)

Quick links