› 8k7a

Electron Microscopy
4.2Å resolution

Cryo-EM structure of nucleotide-bound ComA E647Q mutant with Mg2+

Released:
DOI: 10.2210/pdb8k7a/pdb
PDB entry 8k7a coloured by chain, front view.
PDB entry 8k7a coloured by chain, side view.
PDB entry 8k7a coloured by chain, top view.
Source organism: Streptococcus pneumoniae R6
Primary publication:
Structural basis of peptide secretion for Quorum sensing by ComA.
Yu L, Xu X, Chua WZ, Feng H, Ser Z, Shao K, Shi J, Wang Y, Li Z, Sobota RM, Sham LT, Luo M
OpenAccess logo Nat Commun 14 7178 (2023)
PMID: 37935699
Related structures: EMD-36936

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-230942 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transport/processing ATP-binding protein ComA Chains: A, B
Molecule details ›
Chains: A, B
Length: 717 amino acids
Theoretical weight: 80.43 KDa
Source organism: Streptococcus pneumoniae R6
Expression system: Escherichia coli
UniProt:
  • Canonical: P59653 (Residues: 1-717; Coverage: 100%)
Gene names: comA, spr0043
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand ATP 2 x ATP
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.2Å
Relevant EMDB volumes: EMD-36936
Expression system: Escherichia coli

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