› 8k86

X-ray diffraction
2.06Å resolution

Crystal structure of NFIL3 in complex with TTATGTAA DNA

Released:
DOI: 10.2210/pdb8k86/pdb
PDB entry 8k86 coloured by chain, front view.
PDB entry 8k86 coloured by chain, side view.
PDB entry 8k86 coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis for specific DNA sequence recognition by the transcription factor NFIL3.
Chen S, Lei M, Liu K, Min J
OpenAccess logo J Biol Chem 105776 (2024)
PMID: 38382670

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-240736 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Nuclear factor interleukin-3-regulated protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 73 amino acids
Theoretical weight: 8.49 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q16649 (Residues: 68-136; Coverage: 15%)
Gene names: E4BP4, IL3BP1, NFIL3
Sequence domains: Basic region leucine zipper
DNA (5'-D(*CP*AP*TP*TP*AP*TP*GP*TP*AP*AP*CP*G)-3') Chain: C
Molecule details ›
Chain: C
Length: 12 nucleotides
Theoretical weight: 3.66 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA (5'-D(*CP*GP*TP*TP*AP*CP*AP*TP*AP*AP*TP*G)-3') Chain: D
Molecule details ›
Chain: D
Length: 12 nucleotides
Theoretical weight: 3.66 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P21212
Unit cell:
a: 74.084Å b: 97.52Å c: 39.007Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.227 0.223 0.259
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

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