› 8k8c

X-ray diffraction
2.06Å resolution

Crystal structure of C/EBPalpha BZIP domain bound to a high affinity DNA

Released:
DOI: 10.2210/pdb8k8c/pdb
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PDB entry 8k8c coloured by chain, front view.
PDB entry 8k8c coloured by chain, side view.
PDB entry 8k8c coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis for specific DNA sequence recognition by the transcription factor NFIL3.
Chen S, Lei M, Liu K, Min J
OpenAccess logo J Biol Chem 105776 (2024)
PMID: 38382670

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-270583 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
CCAAT/enhancer-binding protein alpha Chains: A, B
Molecule details ›
Chains: A, B
Length: 61 amino acids
Theoretical weight: 7.34 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P49715 (Residues: 281-340; Coverage: 17%)
Gene names: CEBP, CEBPA
Sequence domains: Basic region leucine zipper
DNA (5'-D(*CP*AP*TP*TP*AP*CP*GP*TP*AP*AP*TP*GP*A)-3') Chain: C
Molecule details ›
Chain: C
Length: 13 nucleotides
Theoretical weight: 3.97 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA (5'-D(*CP*AP*TP*TP*AP*CP*GP*TP*AP*AP*TP*GP*T)-3') Chain: D

Ligands and Environments

1 bound ligand:
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P212121
Unit cell:
a: 39.382Å b: 63.366Å c: 128.546Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.231 0.226 0.282
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

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