› 8k9i

Electron Microscopy
4.2Å resolution

Structure of CUL3-RBX1-KLHL22 complex without CUL3 NA motif

Released:
DOI: 10.2210/pdb8k9i/pdb
PDB entry 8k9i coloured by chain, front view.
PDB entry 8k9i coloured by chain, side view.
PDB entry 8k9i coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
A conserved N-terminal motif of CUL3 contributes to assembly and E3 ligase activity of CRL3KLHL22.
Wang W, Liang L, Dai Z, Zuo P, Yu S, Lu Y, Ding D, Chen H, Shan H, Jin Y, Mao Y, Yin Y
OpenAccess logo Nat Commun 15 3789 (2024)
PMID: 38710693
Related structures: EMD-36987

Function and Biology Details

Reactions catalysed: 
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
S-[NEDD8-protein]-yl-[E2 NEDD8-conjugating enzyme]-L-cysteine + [cullin]-L-lysine = [E2 NEDD8-conjugating enzyme]-L-cysteine + N(6)-[NEDD8-protein]-yl-[cullin]-L-lysine
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-244824 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Cullin-3 Chain: C
Molecule details ›
Chain: C
Length: 752 amino acids
Theoretical weight: 87.43 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q13618 (Residues: 25-768; Coverage: 97%)
Gene names: CUL3, KIAA0617
Sequence domains:
Kelch-like protein 22 Chains: K, L
Molecule details ›
Chains: K, L
Length: 178 amino acids
Theoretical weight: 20.37 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q53GT1 (Residues: 1-178; Coverage: 28%)
Gene name: KLHL22
Sequence domains: BTB/POZ domain
E3 ubiquitin-protein ligase RBX1 Chain: R
Molecule details ›
Chain: R
Length: 108 amino acids
Theoretical weight: 12.29 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P62877 (Residues: 1-108; Coverage: 100%)
Gene names: RBX1, RNF75, ROC1
Sequence domains: RING-H2 zinc finger domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.2Å
Relevant EMDB volumes: EMD-36987
Expression system: Spodoptera frugiperda

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