Function and Biology Details
Biochemical function:
Biological process:
Cellular component:
- not assigned
Structure analysis Details
Assembly composition:
hetero pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-245006 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
2 distinct RNA molecules
2 distinct DNA molecules
2 distinct RNA molecules
Macromolecules (5 distinct):
CRISPR-associated endonuclease Cas9/Csn1
Molecule details ›
Chains: B, F
Length: 1368 amino acids
Theoretical weight: 158.59 KDa
Source organism: Streptococcus pyogenes serotype M1
Expression system: Escherichia coli BL21(DE3)
UniProt:
Sequence domains:
Length: 1368 amino acids
Theoretical weight: 158.59 KDa
Source organism: Streptococcus pyogenes serotype M1
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical:
Q99ZW2 (Residues: 1-1368; Coverage: 100%)
Sequence domains:
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
X-ray source:
SSRF BEAMLINE BL18U1
Spacegroup:
P21
Expression systems:
- Escherichia coli BL21(DE3)
- Not provided
- Escherichia coli DH5[alpha]
