› 8kcl

X-ray diffraction
1.47Å resolution

Crystal Structure of M- and C-Domains of the shaft pilin LrpA from Ligilactobacillus ruminis - Triclinic form

Released:
DOI: 10.2210/pdb8kcl/pdb
PDB entry 8kcl coloured by chain, front view.
PDB entry 8kcl coloured by chain, side view.
PDB entry 8kcl coloured by chain, top view.
Source organism: Ligilactobacillus ruminis ATCC 25644
Primary publication:
The crystal structure of the N-terminal domain of the backbone pilin LrpA reveals a new closure-and-twist motion for assembling dynamic pili in Ligilactobacillus ruminis.
Prajapati A, Palva A, von Ossowski I, Krishnan V
Acta Crystallogr D Struct Biol (2024)
PMID: 38935340

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-246871 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Gram-positive cocci surface proteins LPxTG domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 259 amino acids
Theoretical weight: 28.14 KDa
Source organism: Ligilactobacillus ruminis ATCC 25644
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: E7FRT5 (Residues: 208-466; Coverage: 55%)
Gene name: HMPREF0542_11612
Sequence domains: Prealbumin-like fold domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ELETTRA BEAMLINE 11.2C
Spacegroup: P1
Unit cell:
a: 36.334Å b: 47.677Å c: 51.72Å
α: 107.25° β: 101.52° γ: 111.78°
R-values:
R R work R free
0.175 0.175 0.191
Expression system: Escherichia coli BL21(DE3)

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