› › Structure analysis

Cryo-EM structure of human gamma-secretase in complex with MK-0752

Electron Microscopy
2.7Å resolution
PDB entry 8kcu coloured by chain, front view.
PDB entry 8kcu coloured by chain, side view.
PDB entry 8kcu coloured by chain, top view.
Source organism: Homo sapiens
Assembly composition:
hetero tetramer (preferred)
Entry contents: 4 distinct polypeptide molecules

Quick links

Assemblies

Assembly 1 (preferred)
Download    3D Visualisation
Assembly Name: Gamma-secretase complex, APH1A-PSEN1 variant
hide full assembly name
Multimeric state: hetero tetramer
Accessible surface area: 59801.57 Å2
Buried surface area: 19627.6 Å2
Dissociation area: 1,644.11 Å2
Dissociation energy (ΔGdiss): 19.15 kcal/mol
Dissociation entropy (TΔSdiss): 12.75 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-156020

Macromolecules

Presenilin-1
Chain: B
Length: 467 amino acids
Theoretical weight: 52.71 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P49768 (Residues: 1-467; Coverage: 100%)
Gene names: AD3, PS1, PSEN1, PSNL1
Pfam: Presenilin
Presenilin-1 in PDB entry 8kcu, assembly 1, front view.
Presenilin-1 in PDB entry 8kcu, assembly 1, side view.
Presenilin-1 in PDB entry 8kcu, assembly 1, top view.
PDBe-KB: UniProt Coverage View: P49768  
146750100150200250300350400450
 
200400MTELPAPLSYFQNAQMSEDNHLSNTVRSQNDNRERQEHNDRRSLGHPEPLSNGRPQGNSRQVVEQDEEEDEELTLKYGAKHVIMLFVPVTLCMVVVVATIKSVSFYTRKDGQLIYTPFTEDTETVGQRALHSILNAAIMISVIVVMTILLVVLYKYRCYKVIHAWLIISSLLLLFFFSFIYLGEVFKTYNVAVDYITVALLIWNFGVVGMISIHWKGPLRLQQAYLIMISALMALVFIKYLPEWTAWLILAVISVYDLVAVLCPKGPLRMLVETAQERNETLFPALIYSSTMVWLVNMAEGDPEAQRRVSKNSKYNAESTERESQDTVAENDDGGFSEEWEAQRDSHLGPHRSTPESRAAVQELSSSILAGEDPEERGVKLGLGDFIFYSVLVGKASATASGDWNTTIACFVAILIGLCLTLLLLAIFKKALPALPISITFGLVFYFATDYLVQPFMDQLAFHQFYI
UniProt
P49768
PRO_0000025591
PRO_0000025592
PRO_0000236055
Chains
Secondary structure
Flexibility predictions
Ligand binding sites
Interaction interfaces
Sequence conservation
Variants
Molecule details ›

Search similar proteins

Nicastrin
Chain: A
Length: 701 amino acids
Theoretical weight: 77.62 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q92542 (Residues: 1-701; Coverage: 99%)
Gene names: KIAA0253, NCSTN, UNQ1874/PRO4317
Pfam:
Nicastrin in PDB entry 8kcu, assembly 1, front view.
Nicastrin in PDB entry 8kcu, assembly 1, side view.
Nicastrin in PDB entry 8kcu, assembly 1, top view.
PDBe-KB: UniProt Coverage View: Q92542  
170150100150200250300350400450500550600650700
 
200400600MATAGGGSGADPGSRGLLRLLSFCVLLAGLCRGNSVERKIYIPLNKTAPCVRLLNATHQIGCQSSISGDTGVIHVVEKEEDLQWVLTDGPNPPYMVLLESKHFTRDLMEKLKGRTSRIAGLAVSLTKPSPASGFSPSVQCPNDGFGVYSNSYGPEFAHCREIQWNSLGNGLAYEDFSFPIFLLEDENETKVIKQCYQDHNLSQNGSAPTFPLCAMQLFSHMHAVISTATCMRRSSIQSTFSINPEIVCDPLSDYNVWSMLKPINTTGTLKPDDRVVVAATRLDSRSFFWNVAPGAESAVASFVTQLAAAEALQKAPDVTTLPRNVMFVFFQGETFDYIGSSRMVYDMEKGKFPVQLENVDSFVELGQVALRTSLELWMHTDPVSQKNESVRNQVEDLLATLEKSGAGVPAVILRRPNQSQPLPPSSLQRFLRARNISGVVLADHSGAFHNKYYQSIYDTAENINVSYPEWLSPEEDLNFVTDTAKALADVATVLGRALYELAGGTNFSDTVQADPQTVTRLLYGFLIKANNSWFQSILRQDLRSYLGDGPLQHYIAVSSPTNTTYVVQYALANLTGTVVNLTREQCQDPSKVPSENKDLYEYSWVQGPLHSNETDRLPRCVRSTARLARALSPAFELSQWSSTEYSTWTESRWKDIRARIFLIASKELELITLTVGFGILIFSLIVTYCINAKADVLFIAP
UniProt
Q92542
Chains
Secondary structure
Ligand binding sites
Interaction interfaces
Sequence conservation
Variants
Molecule details ›

Search similar proteins

Gamma-secretase subunit APH-1A
Chain: C
Length: 265 amino acids
Theoretical weight: 29.02 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q96BI3 (Residues: 1-265; Coverage: 100%)
Gene names: APH1A, CGI-78, PSF, UNQ579/PRO1141
Pfam: Aph-1 protein
Gamma-secretase subunit APH-1A in PDB entry 8kcu, assembly 1, front view.
Gamma-secretase subunit APH-1A in PDB entry 8kcu, assembly 1, side view.
Gamma-secretase subunit APH-1A in PDB entry 8kcu, assembly 1, top view.
PDBe-KB: UniProt Coverage View: Q96BI3  
126520406080100120140160180200220240260
 
100200MGAAVFFGCTFVAFGPAFALFLITVAGDPLRVIILVAGAFFWLVSLLLASVVWFILVHVTDRSDARLQYGLLIFGAAVSVLLQEVFRFAYYKLLKKADEGLASLSEDGRSPISIRQMAYVSGLSFGIISGVFSVINILADALGPGVVGIHGDSPYYFLTSAFLTAAIILLHTFWGVVFFDACERRRYWALGLVVGSHLLTSGLTFLNPWYEASLLPIYAVTVSMGLWAFITAGGSLRSIQRSLLCRRQEDSRVMVYSALRIPPED
UniProt
Q96BI3
Chains
Secondary structure
Ligand binding sites
Interaction interfaces
Sequence conservation
Variants
Molecule details ›

Search similar proteins

Gamma-secretase subunit PEN-2
Chain: D
Length: 101 amino acids
Theoretical weight: 12.04 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q9NZ42 (Residues: 1-101; Coverage: 100%)
Gene names: MDS033, PEN2, PSENEN
Pfam: Presenilin enhancer-2 subunit of gamma secretase
Gamma-secretase subunit PEN-2 in PDB entry 8kcu, assembly 1, front view.
Gamma-secretase subunit PEN-2 in PDB entry 8kcu, assembly 1, side view.
Gamma-secretase subunit PEN-2 in PDB entry 8kcu, assembly 1, top view.
PDBe-KB: UniProt Coverage View: Q9NZ42  
1101102030405060708090100
 
50100MNLERVSNEEKLNLCRKYYLGGFAFLPFLWLVNIFWFFREAFLVPAYTEQSQIKGYVWRSAVGFLFWVIVLTSWITIFQIYRPRWGALGDYLSFTIPLGTP
UniProt
Q9NZ42
Chains
Secondary structure
Interaction interfaces
Sequence conservation
Variants
Molecule details ›

Search similar proteins

Quick links