› 8kdc

Electron Microscopy
3.3Å resolution

Cryo-EM structure of the human parainfluenza virus hPIV3 L-P polymerase in monomeric form

Released:
DOI: 10.2210/pdb8kdc/pdb
PDB entry 8kdc coloured by chain, front view.
PDB entry 8kdc coloured by chain, side view.
PDB entry 8kdc coloured by chain, top view.
Source organism: Human respirovirus 3
Primary publication:
Structural basis for dimerization of a paramyxovirus polymerase complex.
Xie J, Ouizougun-Oubari M, Wang L, Zhai G, Wu D, Lin Z, Wang M, Ludeke B, Yan X, Nilsson T, Gao L, Huang X, Fearns R, Chen S
OpenAccess logo Nat Commun 15 3163 (2024)
PMID: 38605025
Related structures: EMD-37131

Function and Biology Details

Reactions catalysed: 
(1a) S-adenosyl-L-methionine + G(5')pppAACA-[mRNA] = S-adenosyl-L-homocysteine + G(5')pppAmACA-[mRNA]
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
(1a) (5')pppAACA-[mRNA] + (protein L)-L-histidine = diphosphate + (protein L)-L-histidyl-(5')phosphonato-AACA-[mRNA] + H(2)O
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-271879 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
RNA-directed RNA polymerase L Chain: A
Molecule details ›
Chain: A
Length: 2266 amino acids
Theoretical weight: 260.1 KDa
Source organism: Human respirovirus 3
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: O89238 (Residues: 1-2258; Coverage: 100%)
Sequence domains:
Phosphoprotein Chains: B, C, D, E, F
Molecule details ›
Chains: B, C, D, E, F
Length: 609 amino acids
Theoretical weight: 68.47 KDa
Source organism: Human respirovirus 3
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: O89234 (Residues: 1-603; Coverage: 100%)
Sequence domains: Paramyxovirinae P phosphoprotein C-terminal region

Ligands and Environments

2 bound ligands:
Ligand MG 1 x MG
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.3Å
Relevant EMDB volumes: EMD-37131
Expression system: Spodoptera frugiperda

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