› 8kek

Electron Microscopy
3.54Å resolution

Monomer state of SARS-CoV Spike protein complexed with antibody PW5-535

Released:
DOI: 10.2210/pdb8kek/pdb
PDB entry 8kek coloured by chain, front view.
PDB entry 8kek coloured by chain, side view.
PDB entry 8kek coloured by chain, top view.
Source organisms:
Primary publication:
Potent and broadly neutralizing antibodies against sarbecoviruses induced by sequential COVID-19 vaccination.
Zhao X, Qiu T, Huang X, Mao Q, Wang Y, Qiao R, Li J, Mao T, Wang Y, Cun Y, Wang C, Luo C, Yoon C, Wang X, Li C, Cui Y, Zhao C, Li M, Chen Y, Cai G, Geng W, Hu Z, Cao J, Zhang W, Cao Z, Chu H, Sun L, Wang P
Cell Discov 10 14 (2024)
PMID: 38320990
Related structures: EMD-37161

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-250283 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
PW5-535 heavy chain Chain: H
Molecule details ›
Chain: H
Length: 450 amino acids
Theoretical weight: 49.38 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
PW5-535 light chain Chain: L
Molecule details ›
Chain: L
Length: 215 amino acids
Theoretical weight: 23.46 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Spike glycoprotein Chain: A
Molecule details ›
Chain: A
Length: 1190 amino acids
Theoretical weight: 131.98 KDa
Source organism: Severe acute respiratory syndrome coronavirus 2
Expression system: Homo sapiens
UniProt:
  • Canonical: P59594 (Residues: 1-1190; Coverage: 95%)
Gene names: 2, S
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.54Å
Relevant EMDB volumes: EMD-37161
Expression system: Homo sapiens

Quick links

Citations

3 citation in other articles

Bispecific antibodies provide broad neutralization of emerging beta-coronaviruses by targeting ACE2 and viral spikes.
Li et al. (2024)
2 more