› 8kfj

X-ray diffraction
1.9Å resolution

Crystal structure of sperm whale myoglobin (F46L/H64A mutant) reconstituted with manganese porphycene

Released:
DOI: 10.2210/pdb8kfj/pdb
PDB entry 8kfj coloured by chain, front view.
PDB entry 8kfj coloured by chain, side view.
PDB entry 8kfj coloured by chain, top view.
Source organism: Physeter catodon
Entry authors: Mizohata E, Oohora K, Hayashi T

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-135409 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Myoglobin Chains: A, B
Molecule details ›
Chains: A, B
Length: 170 amino acids
Theoretical weight: 19.29 KDa
Source organism: Physeter catodon
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P02185 (Residues: 2-154; Coverage: 99%)
Gene name: MB
Sequence domains: Globin

Ligands and Environments

3 bound ligands:
Ligand HNN 2 x HNN
Ligand SO4 13 x SO4
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: P212121
Unit cell:
a: 56.673Å b: 80.174Å c: 106.595Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.18 0.179 0.212
Expression system: Escherichia coli BL21(DE3)

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