› 8kgk

Electron Microscopy
3.16Å resolution

Cryo-EM structure of the GPR61-Gs complex

Released:
DOI: 10.2210/pdb8kgk/pdb
PDB entry 8kgk coloured by chain, front view.
PDB entry 8kgk coloured by chain, side view.
PDB entry 8kgk coloured by chain, top view.
Source organisms:
Primary publication:
Specific binding of GPR174 by endogenous lysophosphatidylserine leads to high constitutive Gs signaling.
Nie Y, Qiu Z, Chen S, Chen Z, Song X, Ma Y, Huang N, Cyster JG, Zheng S
OpenAccess logo Nat Commun 14 5901 (2023)
PMID: 37737235
Related structures: EMD-37224

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-231803 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
G-protein coupled receptor 61 Chain: A
Molecule details ›
Chain: A
Length: 390 amino acids
Theoretical weight: 42.53 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q9BZJ8 (Residues: 1-365; Coverage: 81%)
Gene names: BALGR, GPCR3, GPR61
Sequence domains: 7 transmembrane receptor (rhodopsin family)
Guanine nucleotide-binding protein G(olf) subunit alpha; Guanine nucleotide-binding protein G(s) subunit alpha isoforms short Chain: B
Molecule details ›
Chain: B
Length: 249 amino acids
Theoretical weight: 29.01 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P63092 (Residues: 6-9, 13-64, 204-394; Coverage: 60%)
  • Canonical: P38405 (Residues: 11-14; Coverage: 1%)
Gene names: GNAL, GNAS, GNAS1, GSP
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: C
Molecule details ›
Chain: C
Length: 357 amino acids
Theoretical weight: 39.29 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P62873 (Residues: 2-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: D
Molecule details ›
Chain: D
Length: 71 amino acids
Theoretical weight: 7.85 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P59768 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain
Nanobody 35 Chain: E
Molecule details ›
Chain: E
Length: 160 amino acids
Theoretical weight: 17.35 KDa
Source organism: Lama glama
Expression system: Escherichia coli BL21(DE3)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.16Å
Relevant EMDB volumes: EMD-37224
Expression systems:
  • Homo sapiens
  • Spodoptera frugiperda
  • Escherichia coli BL21(DE3)

Quick links

Citations

3 citation in other articles

GPR161 structure uncovers the redundant role of sterol-regulated ciliary cAMP signaling in the Hedgehog pathway.
Hoppe et al. (2024)
2 more