› 8kgr

Electron Microscopy
3.2Å resolution

Structure of African swine fever virus topoisomerase II in complex with dsDNA

Released:
DOI: 10.2210/pdb8kgr/pdb
PDB entry 8kgr coloured by chain, front view.
PDB entry 8kgr coloured by chain, side view.
PDB entry 8kgr coloured by chain, top view.
Source organisms:
Primary publication:
Structural insights into the DNA topoisomerase II of the African swine fever virus.
Cong J, Xin Y, Kang H, Yang Y, Wang C, Zhao D, Li X, Rao Z, Chen Y
Nat Commun 15 4607 (2024)
PMID: 38816407
Related structures: EMD-37231

Function and Biology Details

Reaction catalysed: 
ATP-dependent breakage, passage and rejoining of double-stranded DNA
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-269186 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA topoisomerase 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 1211 amino acids
Theoretical weight: 138.09 KDa
Source organism: African swine fever virus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: A0A2X0THW2 (Residues: 1-1192; Coverage: 100%)
Gene names: ASFV-Georgia_4-136, P1192R, P1192R CDS
Sequence domains:
DNA (32-MER) Chain: C
Molecule details ›
Chain: C
Length: 52 nucleotides
Theoretical weight: 16.06 KDa
Source organism: DNA molecule
Expression system: Not provided
DNA (33-MER) Chain: D
Molecule details ›
Chain: D
Length: 52 nucleotides
Theoretical weight: 15.97 KDa
Source organism: DNA molecule
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.2Å
Relevant EMDB volumes: EMD-37231
Expression systems:
  • Spodoptera frugiperda
  • Not provided

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