› 8ki7

Electron Microscopy
3.7Å resolution

Structure of Tomato spotted wilt virus L protein contained endoH domain binding to 3'5'vRNA

Released:
DOI: 10.2210/pdb8ki7/pdb
PDB entry 8ki7 coloured by chain, front view.
PDB entry 8ki7 coloured by chain, side view.
PDB entry 8ki7 coloured by chain, top view.
Source organisms:
Entry authors: Cao L, Wang X
Related structures: EMD-37253

Function and Biology Details

Reaction catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-281152 (preferred)
Entry contents:
1 distinct polypeptide molecule
3 distinct RNA molecules
Macromolecules (4 distinct):
RNA-directed RNA polymerase L Chain: A
Molecule details ›
Chain: A
Length: 2090 amino acids
Theoretical weight: 240.38 KDa
Source organism: Tomato spotted wilt virus (strain Bulgarian L3)
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: A0A7G8JUQ8 (Residues: 1-2090; Coverage: 73%)
Sequence domains: Bunyavirus RNA dependent RNA polymerase
RNA (5'-R(P*AP*GP*AP*GP*CP*AP*AP*UP*CP*A)-3') Chain: E
Molecule details ›
Chain: E
Length: 10 nucleotides
Theoretical weight: 3.21 KDa
Source organism: synthetic construct
Expression system: Not provided
RNA (5'-R(P*GP*CP*AP*AP*UP*CP*AP*GP*G)-3') Chain: B
Molecule details ›
Chain: B
Length: 9 nucleotides
Theoretical weight: 2.89 KDa
Source organism: synthetic construct
Expression system: Not provided
RNA (5'-R(P*AP*CP*CP*UP*GP*AP*UP*UP*GP*CP*UP*CP*U)-3') Chain: F
Molecule details ›
Chain: F
Length: 13 nucleotides
Theoretical weight: 4.06 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.7Å
Relevant EMDB volumes: EMD-37253
Expression systems:
  • Spodoptera frugiperda
  • Not provided

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