› 8nse

X-ray diffraction
2.25Å resolution

BOVINE ENDOTHELIAL NITRIC OXIDE SYNTHASE, NNA COMPLEX

Released:
DOI: 10.2210/pdb8nse/pdb
PDB entry 8nse coloured by chain, front view.
PDB entry 8nse coloured by chain, side view.
PDB entry 8nse coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Crystal structure of nitric oxide synthase bound to nitro indazole reveals a novel inactivation mechanism.
Raman CS, Li H, Martásek P, Southan G, Masters BS, Poulos TL
Biochemistry 40 13448-55 (2001)
PMID: 11695891

Function and Biology Details

Reaction catalysed: 
(1a) 2 L-arginine + 2 NADPH + 2 O(2) = 2 N(omega)-hydroxy-L-arginine + 2 NADP(+) + 2 H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151510 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nitric oxide synthase 3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 444 amino acids
Theoretical weight: 49.71 KDa
Source organism: Bos taurus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P29473 (Residues: 39-482; Coverage: 37%)
Gene name: NOS3
Sequence domains: Nitric oxide synthase, oxygenase domain
Structure domains:

Ligands and Environments


Cofactor: Ligand HEM 2 x HEM
5 bound ligands:
Ligand ZN 1 x ZN
Ligand H4B 2 x H4B
Ligand NRG 2 x NRG
Ligand CAD 2 x CAD
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F2
Spacegroup: P212121
Unit cell:
a: 58.31Å b: 105.78Å c: 156.61Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.227 0.227 0.276
Expression system: Escherichia coli BL21

Quick links

Citations

8 review citations

Implications of protein flexibility for drug discovery.
Teague SJ. (2003)
7 more

2 mentions without citation

Identification of redox partners and development of a novel chimeric bacterial nitric oxide synthase for structure activity analyses.
Holden et al. (2014)
1 more