› 8odx

X-ray diffraction
4.4Å resolution

Interleukin 12 receptor subunit beta-1 Fn domains in complex with antagonistic FAb4 fragment and VHH.

Released:
DOI: 10.2210/pdb8odx/pdb
PDB entry 8odx coloured by chain, front view.
PDB entry 8odx coloured by chain, side view.
PDB entry 8odx coloured by chain, top view.
Source organisms:
Primary publication:
Structures of complete extracellular receptor assemblies mediated by IL-12 and IL-23.
Bloch Y, Felix J, Merceron R, Provost M, Symakani RA, De Backer R, Lambert E, Mehdipour AR, Savvides SN
Nat Struct Mol Biol (2024)
PMID: 38287195

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-239683 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Interleukin-12 receptor subunit beta-1 Chain: A
Molecule details ›
Chain: A
Length: 376 amino acids
Theoretical weight: 40.88 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P42701 (Residues: 232-540; Coverage: 48%)
  • Best match: P42701-3 (Residues: 232-341)
Gene names: IL12R, IL12RB, IL12RB1
Sequence domains: Fibronectin type III domain
FAb4 Heavy chain Chain: H
Molecule details ›
Chain: H
Length: 250 amino acids
Theoretical weight: 26.78 KDa
Source organism: Homo sapiens x Mus musculus hybrid cell line
Expression system: Homo sapiens
anti Kappa Light chain VHH Chain: K
Molecule details ›
Chain: K
Length: 167 amino acids
Theoretical weight: 18.06 KDa
Source organism: Lama glama
Expression system: Escherichia coli
FAb4 Light chain Chain: L
Molecule details ›
Chain: L
Length: 231 amino acids
Theoretical weight: 25.2 KDa
Source organism: Homo sapiens x Mus musculus hybrid cell line
Expression system: Homo sapiens

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Spacegroup: P4132
Unit cell:
a: 231.402Å b: 231.402Å c: 231.402Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.283 0.28 0.312
Expression systems:
  • Homo sapiens
  • Escherichia coli

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