› 8oeq

X-ray diffraction
3.3Å resolution

Crystal structure of the Candida albicans 80S ribosome in complex with Paromomycin (250uM)

Released:
DOI: 10.2210/pdb8oeq/pdb
Model geometry
Fit model/data
PDB entry 8oeq coloured by chain, front view.
PDB entry 8oeq coloured by chain, side view.
PDB entry 8oeq coloured by chain, top view.
Source organism: Candida albicans
Entry authors: Kolosova O, Zgadzay Y, Yusupov M

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 80-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-292191 (preferred)
Entry contents:
76 distinct polypeptide molecules
4 distinct RNA molecules
Macromolecules (80 distinct):
Large ribosomal subunit protein uL2 C-terminal domain-containing protein Chains: AW, j
Molecule details ›
Chains: AW, j
Length: 254 amino acids
Theoretical weight: 27.37 KDa
Source organism: Candida albicans
UniProt:
  • Canonical: A0A8H6F1X9 (Residues: 1-254; Coverage: 100%)
Gene names: FOB64_005652, RPL2B
Sequence domains:
Large ribosomal subunit protein uL3 Chains: AX, k
Molecule details ›
Chains: AX, k
Length: 389 amino acids
Theoretical weight: 44.04 KDa
Source organism: Candida albicans
UniProt:
  • Canonical: Q59LS1 (Residues: 1-389; Coverage: 100%)
Gene names: CAALFM_C209430WA, RPL3, orf19.1601
Sequence domains: Ribosomal protein L3
Large ribosomal subunit protein uL4 C-terminal domain-containing protein Chains: AY, l
Large ribosomal subunit protein uL18 C-terminal eukaryotes domain-containing protein Chains: AZ, m
60S ribosomal protein L6 Chains: BA, n
60S ribosomal protein L7 Chains: BB, o
60S ribosomal protein L8 Chains: BC, p
Large ribosomal subunit protein uL6 alpha-beta domain-containing protein Chains: BD, q
Large ribosomal subunit protein uL16 Chains: BE, r
60S ribosomal protein L11 Chains: BF, s
60S ribosomal protein L13 Chains: BG, t
Large ribosomal subunit protein eL14 domain-containing protein Chains: BH, u
Large ribosomal subunit protein eL15 Chains: BI, v
60S ribosomal protein L16-A Chains: BJ, w
60S ribosomal protein L17-B Chains: BK, x
Large ribosomal subunit protein uL15/eL18 domain-containing protein Chains: BL, y
Large ribosomal subunit protein eL19 Chains: BM, z
60S ribosomal protein L20 Chains: 0, BN
60S ribosomal protein L21-A Chains: 2, BO
Large ribosomal subunit protein eL22 Chains: 5, BP
60S ribosomal protein L23-A Chains: 6, BQ
Large ribosomal subunit protein eL24-related N-terminal domain-containing protein Chains: 7, BR
Large ribosomal subunit protein uL23 N-terminal domain-containing protein Chains: 8, BS
Ribosomal 60S subunit protein L26A Chains: 9, BT
Large ribosomal subunit protein eL27 Chains: AA, BU
Large ribosomal subunit protein uL15/eL18 domain-containing protein Chains: AB, BV
60S ribosomal protein L29 Chains: AC, BW
Large ribosomal subunit protein eL30 Chains: AD, BX
Ribosomal 60S subunit protein L31B Chains: AE, BY
Large ribosomal subunit protein eL32 Chains: AF, BZ
Ribosomal 60S subunit protein L33A Chains: AG, CA
60S ribosomal protein L34-B Chains: AH, CB
Ribosomal 60S subunit protein L35A Chains: AI, CC
Large ribosomal subunit protein eL36 Chains: AJ, CD
Large ribosomal subunit protein eL37 Chains: AK, CE
Large ribosomal subunit protein eL38 Chains: AL, CF
Large ribosomal subunit protein eL39 Chains: AM, CG
Large ribosomal subunit protein eL40 Chains: AN, CH
Small ribosomal subunit protein eS32 Chains: AO, CI
60S ribosomal protein L44 Chains: AP, CJ
Large ribosomal subunit protein eL43 Chains: AQ, CK
Ribosome-associated protein STM1 Chains: CL, i
Small ribosomal subunit protein uS2 Chains: C, CN
Small ribosomal subunit protein eS1 Chains: CO, D
Small ribosomal subunit protein uS5 Chains: CP, E
40S ribosomal protein S3 Chains: CQ, F
Small ribosomal subunit protein eS4 Chains: CR, G
Small ribosomal subunit protein uS7 domain-containing protein Chains: CS, H
40S ribosomal protein S6 Chains: CT, I
40S ribosomal protein S7 Chains: CU, J
40S ribosomal protein S8 Chains: CV, K
40S ribosomal protein S9-B Chains: CW, L
Plectin/eS10 N-terminal domain-containing protein Chains: CX, M
Small ribosomal subunit protein uS17 Chains: CY, N
40S ribosomal protein S12 Chains: CZ, O
Small ribosomal subunit protein uS15 N-terminal domain-containing protein Chains: DA, P
40S ribosomal protein S14 Chains: DB, Q
Small ribosomal subunit protein uS19 Chains: DC, R
Small ribosomal subunit protein uS9 Chains: DD, S
40S ribosomal protein S17-B Chains: DE, T
40S ribosomal protein S18 Chains: DF, U
40S ribosomal protein S19-A Chains: DG, V
Small ribosomal subunit protein uS10 domain-containing protein Chains: DH, W
Small ribosomal subunit protein eS21 Chains: DI, X
40S ribosomal protein S22 Chains: DJ, Y
40S ribosomal protein S23 Chains: DK, Z
40S ribosomal protein S24 Chains: DL, a
40S ribosomal protein S25 Chains: DM, b
40S ribosomal protein S26 Chains: DN, c
40S ribosomal protein S27 Chains: DO, d
40S ribosomal protein S28 Chains: DP, e
Small ribosomal subunit protein uS14 Chains: DQ, f
Small ribosomal subunit protein eS30 Chains: DR, g
Ubiquitin-ribosomal protein eS31 fusion protein Chains: DS, h
Guanine nucleotide-binding protein subunit beta-like protein Chains: AR, DT
Ribosomal protein Chains: L1, l1
25S ribosomal RNA Chains: 1, AS
5S ribosomal RNA Chains: 3, AT
5.8S ribosomal RNA Chains: 4, AU
18S Ribosomal RNA Chains: B, CM

Ligands and Environments

4 bound ligands:
Ligand MG 1455 x MG
Ligand 3K5 2 x 3K5
Ligand PAR 8 x PAR
Ligand ZN 11 x ZN
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P21
Unit cell:
a: 298.32Å b: 293.37Å c: 451.08Å
α: 90° β: 100.16° γ: 90°
R-values:
R R work R free
0.254 0.254 0.298

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