› 8ofb

X-ray diffraction
2.39Å resolution

Crystal Structure of T. maritima reverse gyrase with a minimal latch, hexagonal form

Released:
DOI: 10.2210/pdb8ofb/pdb
PDB entry 8ofb coloured by chain, front view.
PDB entry 8ofb coloured by chain, side view.
PDB entry 8ofb coloured by chain, top view.
Source organism: Thermotoga maritima MSB8
Primary publication:
Structure of reverse gyrase with a minimal latch that supports ATP-dependent positive supercoiling without specific interactions with the topoisomerase domain.
Mhaindarkar VP, Rasche R, Kümmel D, Rudolph MG, Klostermeier D
OpenAccess logo Acta Crystallogr D Struct Biol (2023)
PMID: 37204816

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-520456 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Reverse gyrase Chain: A
Molecule details ›
Chain: A
Length: 1043 amino acids
Theoretical weight: 121.35 KDa
Source organism: Thermotoga maritima MSB8
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O51934 (Residues: 1-1104; Coverage: 95%)
Gene names: TM_0173, rgy, topR
Sequence domains:

Ligands and Environments

4 bound ligands:
Ligand P6G 1 x P6G
Ligand ZN 2 x ZN
Ligand SO4 5 x SO4
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P6122
Unit cell:
a: 90.833Å b: 90.833Å c: 571.878Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.245 0.243 0.272
Expression system: Escherichia coli BL21(DE3)

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Citations

1 mention without citation

Structure of reverse gyrase with a minimal latch that supports ATP-dependent positive supercoiling without specific interactions with the topoisomerase domain.
Mhaindarkar et al. (2023)