› 8oh4

Electron Microscopy
16.5Å resolution

Subtomogram averaging structure of cofilactin filament inside microtubule lumen of Drosophila S2 cell protrusion.

Released:
DOI: 10.2210/pdb8oh4/pdb
PDB entry 8oh4 coloured by chain, front view.
PDB entry 8oh4 coloured by chain, side view.
PDB entry 8oh4 coloured by chain, top view.
Source organism: Drosophila
Primary publication:
CryoET shows cofilactin filaments inside the microtubule lumen.
Santos CV, Rogers SL, Carter AP
OpenAccess logo bioRxiv (2023)
PMID: 37034688
Related structures: EMD-16877

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero tetradecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-215406 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Actin-5C Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 370 amino acids
Theoretical weight: 41.16 KDa
Source organism: Drosophila
UniProt:
  • Canonical: P10987 (Residues: 7-376; Coverage: 98%)
Gene names: Act5C, CG4027
Sequence domains: Actin
Cofilin/actin-depolymerizing factor homolog Chains: I, J, K, L, M, N
Molecule details ›
Chains: I, J, K, L, M, N
Length: 148 amino acids
Theoretical weight: 17.18 KDa
Source organism: Drosophila
UniProt:
  • Canonical: P45594 (Residues: 1-148; Coverage: 100%)
Gene names: CG4254, Cadf, tsr
Sequence domains: Cofilin/tropomyosin-type actin-binding protein

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 16.5Å
Relevant EMDB volumes: EMD-16877

Quick links

Citations

1 mention without citation

DiffModeler: Large Macromolecular Structure Modeling in Low-Resolution Cryo-EM Maps Using Diffusion Model.
Wang et al. (2024)