› 8ohx

X-ray diffraction
1.95Å resolution

Crystal structure of Beta-glucuronidase from Escherichia coli in complex with siastatin B derived inhibitor

Released:
DOI: 10.2210/pdb8ohx/pdb
PDB entry 8ohx coloured by chain, front view.
PDB entry 8ohx coloured by chain, side view.
PDB entry 8ohx coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Molecular Basis for Inhibition of Heparanases and β-Glucuronidases by Siastatin B.
Chen Y, van den Nieuwendijk AMCH, Wu L, Moran E, Skoulikopoulou F, van Riet V, Overkleeft HS, Davies GJ, Armstrong Z
OpenAccess logo J Am Chem Soc (2023)
PMID: 38118176

Function and Biology Details

Reaction catalysed: 
A beta-D-glucuronoside + H(2)O = D-glucuronate + an alcohol
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-238807 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-glucuronidase Chains: AAA, BBB
Molecule details ›
Chains: AAA, BBB
Length: 601 amino acids
Theoretical weight: 68.38 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: U4Q148 (Residues: 20-618; Coverage: 96%)
Gene names: BN877_II1269, uidA
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand VON 2 x VON
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Unit cell:
a: 126.281Å b: 76.711Å c: 141.018Å
α: 90° β: 102.01° γ: 90°
R-values:
R R work R free
0.224 0.221 0.285
Expression system: Escherichia coli

Quick links