› 8oi4

X-ray diffraction
1.76Å resolution

Metagenomic Beta-galactosidase from Glycoside Hydrolase family GH154

Released:
DOI: 10.2210/pdb8oi4/pdb
PDB entry 8oi4 coloured by chain, front view.
PDB entry 8oi4 coloured by chain, side view.
PDB entry 8oi4 coloured by chain, top view.
Source organism: organismal metagenomes
Primary publication:
Novel β-galactosidase activity and first crystal structure of Glycoside Hydrolase family 154.
Hameleers L, Pijning T, Gray BB, Fauré R, Jurak E
N Biotechnol 80 1-11 (2023)
PMID: 38163476

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-239414 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-galactosidase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 398 amino acids
Theoretical weight: 46.26 KDa
Source organism: organismal metagenomes
Expression system: Escherichia coli BL21(DE3)

Ligands and Environments

2 bound ligands:
Ligand GOL 6 x GOL
Ligand CL 6 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P21
Unit cell:
a: 96.316Å b: 94.581Å c: 108.264Å
α: 90° β: 105.45° γ: 90°
R-values:
R R work R free
0.153 0.152 0.171
Expression system: Escherichia coli BL21(DE3)

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