› 8okv

X-ray diffraction
2.6Å resolution

lipoprotein BT2095 from Bacteroides thetaiotamicron bound to cyanocobalamin CnCbl

Released:
DOI: 10.2210/pdb8okv/pdb
PDB entry 8okv coloured by chain, front view.
PDB entry 8okv coloured by chain, side view.
PDB entry 8okv coloured by chain, top view.
Source organism: Bacteroides thetaiotaomicron VPI-5482
Primary publication:
BtuB TonB-dependent transporters and BtuG surface lipoproteins form stable complexes for vitamin B12 uptake in gut Bacteroides.
Abellon-Ruiz J, Jana K, Silale A, Frey AM, Baslé A, Trost M, Kleinekathöfer U, van den Berg B
OpenAccess logo Nat Commun 14 4714 (2023)
PMID: 37543597

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-229575 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Surface layer protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 333 amino acids
Theoretical weight: 38.65 KDa
Source organism: Bacteroides thetaiotaomicron VPI-5482
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: Q8A5Z1 (Residues: 31-355; Coverage: 92%)
Gene name: BT_2095
Sequence domains: Domain of unknown function (DUF5074)

Ligands and Environments

1 bound ligand:
Ligand CNC 4 x CNC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P212121
Unit cell:
a: 74.77Å b: 131.04Å c: 179.38Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.221 0.218 0.276
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

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