› 8onk

X-ray diffraction
3.4Å resolution

Human insulin in complex with the analytical antibody S1 Fab and the analytical antibody HUI-001 Fab

Released:
DOI: 10.2210/pdb8onk/pdb
PDB entry 8onk coloured by chain, front view.
PDB entry 8onk coloured by chain, side view.
PDB entry 8onk coloured by chain, top view.
Source organisms:
Entry author: Johansson E

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-279428 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
Heavy chain of analytical antibody S1 Fab Chains: C, H
Molecule details ›
Chains: C, H
Length: 223 amino acids
Theoretical weight: 24.22 KDa
Source organism: Mus musculus
Expression system: Homo sapiens
Light chain of analytical antibody S1 Fab Chains: E, L
Molecule details ›
Chains: E, L
Length: 213 amino acids
Theoretical weight: 23.36 KDa
Source organism: Mus musculus
Expression system: Homo sapiens
Insulin A chain Chains: D, I
Molecule details ›
Chains: D, I
Length: 21 amino acids
Theoretical weight: 2.38 KDa
Source organism: Homo sapiens
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P01308 (Residues: 90-110; Coverage: 24%)
Gene name: INS
Insulin B chain Chains: A, B
Molecule details ›
Chains: A, B
Length: 30 amino acids
Theoretical weight: 3.43 KDa
Source organism: Homo sapiens
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P01308 (Residues: 25-54; Coverage: 35%)
Gene name: INS
Sequence domains: Insulin/IGF/Relaxin family
Light chain of analytical antibody HUI-001 Fab Chains: F, J
Molecule details ›
Chains: F, J
Length: 215 amino acids
Theoretical weight: 23.54 KDa
Source organism: Mus musculus
Expression system: Homo sapiens
Heavy chain of analytical antibody HUI-001 Fab Chains: G, K
Molecule details ›
Chains: G, K
Length: 220 amino acids
Theoretical weight: 23.51 KDa
Source organism: Mus musculus
Expression system: Homo sapiens

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: C2
Unit cell:
a: 278.9Å b: 52.326Å c: 179.504Å
α: 90° β: 101.14° γ: 90°
R-values:
R R work R free
0.26 0.258 0.311
Expression systems:
  • Homo sapiens
  • Saccharomyces cerevisiae

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