› 8oos

Electron Microscopy
3.29Å resolution

CryoEM Structure INO80core Hexasome complex ATPase-hexasome refinement state 2

Released:
DOI: 10.2210/pdb8oos/pdb
PDB entry 8oos coloured by chain, front view.
PDB entry 8oos coloured by chain, side view.
PDB entry 8oos coloured by chain, top view.
Source organisms:
Primary publication:
Hexasome-INO80 complex reveals structural basis of noncanonical nucleosome remodeling.
Zhang M, Jungblut A, Kunert F, Hauptmann L, Hoffmann T, Kolesnikova O, Metzner F, Moldt M, Weis F, DiMaio F, Hopfner KP, Eustermann S
Science eadf6287 (2023)
PMID: 37384673
Related structures: EMD-17027

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero nonamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-270617 (preferred)
Entry contents:
5 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (7 distinct):
Chromatin-remodeling ATPase Ino80 Chain: G
Molecule details ›
Chain: G
Length: 1134 amino acids
Theoretical weight: 130.89 KDa
Source organism: Thermochaetoides thermophila
Expression system: Trichoplusia ni
Histone H3.1 Chains: M, Q
Molecule details ›
Chains: M, Q
Length: 135 amino acids
Theoretical weight: 15.31 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P68431 (Residues: 2-136; Coverage: 99%)
Gene names: H3C1, H3C10, H3C11, H3C12, H3C2, H3C3, H3C4, H3C6, H3C7, H3C8, H3FA, H3FB, H3FC HIST1H3C, H3FD, H3FF, H3FH, H3FI, H3FJ, H3FK, H3FL, HIST1H3A, HIST1H3B, HIST1H3D, HIST1H3E, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J
Sequence domains: Core histone H2A/H2B/H3/H4
Histone H4 Chains: N, R
Histone H2A type 1-C Chain: O
Histone H2B type 1-C/E/F/G/I Chain: P
DNA strand 1 Chain: K
DNA Strand 2 Chain: L

Ligands and Environments

3 bound ligands:
Ligand ADP 1 x ADP
Ligand MG 1 x MG
Ligand ALF 1 x ALF
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 3.29Å
Relevant EMDB volumes: EMD-17027
Expression systems:
  • Trichoplusia ni
  • Escherichia coli

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Citations

3 review citations

Energy-driven genome regulation by ATP-dependent chromatin remodellers.
Eustermann et al. (2024)
2 more