› 8oq2

X-ray diffraction
1.91Å resolution

Binding of NADP to a formate dehydrogenase from Starkeya novella.

Released:
DOI: 10.2210/pdb8oq2/pdb
PDB entry 8oq2 coloured by chain, front view.
PDB entry 8oq2 coloured by chain, side view.
PDB entry 8oq2 coloured by chain, top view.
Source organism: Starkeya novella
Entry authors: Partipilo M, Whittaker JJ, Pontillo N, Guskov A, Slotboom DJ

Function and Biology Details

Reaction catalysed: 
Formate + NAD(+) = CO(2) + NADH
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-216570 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Formate dehydrogenase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 381 amino acids
Theoretical weight: 42.02 KDa
Source organism: Starkeya novella
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: D7A8L2 (Residues: 2-381; Coverage: 95%)
Gene name: Snov_3272
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand NAP 6 x NAP
Ligand AZI 14 x AZI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE MASSIF-1
Spacegroup: P61
Unit cell:
a: 132.265Å b: 132.265Å c: 302.952Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.237 0.237 0.275
Expression system: Escherichia coli BL21(DE3)

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