› 8orm

Electron Microscopy
1.9Å resolution

Cryo-EM structure of CAK-THZ1

Released:
DOI: 10.2210/pdb8orm/pdb
PDB entry 8orm coloured by chain, front view.
PDB entry 8orm coloured by chain, side view.
PDB entry 8orm coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
High-resolution cryo-EM of the human CDK-activating kinase for structure-based drug design.
Cushing VI, Koh AF, Feng J, Jurgaityte K, Bondke A, Kroll SHB, Barbazanges M, Scheiper B, Bahl AK, Barrett AGM, Ali S, Kotecha A, Greber BJ
Nat Commun 15 2265 (2024)
PMID: 38480681
Related structures: EMD-17129

Function and Biology Details

Reactions catalysed: 
ATP + a protein = ADP + a phosphoprotein
ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156240 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
CDK-activating kinase assembly factor MAT1 Chain: H
Molecule details ›
Chain: H
Length: 93 amino acids
Theoretical weight: 10.23 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P51948 (Residues: 220-309; Coverage: 29%)
Gene names: CAP35, MAT1, MNAT1, RNF66
Cyclin-H Chain: I
Molecule details ›
Chain: I
Length: 324 amino acids
Theoretical weight: 37.72 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P51946 (Residues: 1-323; Coverage: 100%)
Gene name: CCNH
Sequence domains:
Cyclin-dependent kinase 7 Chain: J
Molecule details ›
Chain: J
Length: 349 amino acids
Theoretical weight: 39.36 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P50613 (Residues: 1-346; Coverage: 100%)
Gene names: CAK, CAK1, CDK7, CDKN7, MO15, STK1
Sequence domains: Protein kinase domain

Ligands and Environments

1 bound ligand:
Ligand V0G 1 x V0G
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 1.9Å
Relevant EMDB volumes: EMD-17129
Expression system: Trichoplusia ni

Quick links