› 8osf

Electron Microscopy
4Å resolution

AAA+ motor subunit ChlI of magnesium chelatase, hexamer conformation A

Released:
DOI: 10.2210/pdb8osf/pdb
PDB entry 8osf coloured by chain, front view.
PDB entry 8osf coloured by chain, side view.
PDB entry 8osf coloured by chain, top view.
Source organism: Nostoc sp. PCC 7120 = FACHB-418
Primary publication:
Conformational variability of cyanobacterial ChlI, the AAA+ motor of magnesium chelatase involved in chlorophyll biosynthesis.
Shvarev D, Scholz AI, Moeller A
OpenAccess logo mBio e0189323 (2023)
PMID: 37737632
Related structures: EMD-17151

Function and Biology Details

Reaction catalysed: 
ATP + protoporphyrin IX + Mg(2+) + H(2)O = ADP + phosphate + Mg-protoporphyrin IX + 2 H(+)
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-228116 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Magnesium-chelatase subunit ChlI Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 380 amino acids
Theoretical weight: 42.13 KDa
Source organism: Nostoc sp. PCC 7120 = FACHB-418
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P58571 (Residues: 2-374; Coverage: 100%)
Gene names: all0152, chlI
Sequence domains:

Ligands and Environments

3 bound ligands:
Ligand ATP 5 x ATP
Ligand MG 5 x MG
Ligand ADP 1 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4Å
Relevant EMDB volumes: EMD-17151
Expression system: Escherichia coli BL21(DE3)

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