› 8osn

X-ray diffraction
1.8Å resolution

GTPASE HRAS IN COMPLEX WITH ZN-CYCLEN AT AMBIENT PRESSURE

Released:
DOI: 10.2210/pdb8osn/pdb
PDB entry 8osn coloured by chain, front view.
PDB entry 8osn coloured by chain, side view.
PDB entry 8osn coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
High pressure promotes binding of the allosteric inhibitor Zn2+-cyclen in crystals of activated H-Ras.
Girard E, Lopes P, Spoerner M, Dhaussy AC, Prangé T, Kalbitzer HR, Colloc'h N
Chemistry e202400304 (2024)
PMID: 38647362

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133956 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GTPase HRas, N-terminally processed Chain: A
Molecule details ›
Chain: A
Length: 166 amino acids
Theoretical weight: 18.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01112 (Residues: 1-166; Coverage: 88%)
Gene names: HRAS, HRAS1
Sequence domains: Ras family

Ligands and Environments

3 bound ligands:
Ligand GNP 1 x GNP
Ligand MG 1 x MG
Ligand PGE 1 x PGE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE CRISTAL
Spacegroup: P3221
Unit cell:
a: 39.511Å b: 39.511Å c: 160.84Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.178 0.176 0.215
Expression system: Escherichia coli

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