› 8osw

X-ray diffraction
1.3Å resolution

Crystal structure of Rhizobium etli L-asparaginase ReAIV (R4mC-1)

Released:
DOI: 10.2210/pdb8osw/pdb
PDB entry 8osw coloured by chain, front view.
PDB entry 8osw coloured by chain, side view.
PDB entry 8osw coloured by chain, top view.
Source organism: Rhizobium etli
Primary publication:
Rhizobium etli has two L-asparaginases with low sequence identity but similar structure and catalytic center.
Loch JI, Worsztynowicz P, Sliwiak J, Grzechowiak M, Imiolczyk B, Pokrywka K, Chwastyk M, Gilski M, Jaskolski M
Acta Crystallogr D Struct Biol 79 775-791 (2023)
PMID: 37494066

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-226236 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
L-asparaginase II protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 341 amino acids
Theoretical weight: 36.12 KDa
Source organism: Rhizobium etli
Expression system: Escherichia coli
UniProt:
  • Canonical: Q2KB35 (Residues: 1-335; Coverage: 100%)
Gene name: RHE_CH01144
Sequence domains: L-asparaginase II

Ligands and Environments

2 bound ligands:
Ligand ZN 2 x ZN
Ligand CL 4 x CL
1 modified residue:
Ligand CSO 2 x CSO

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Spacegroup: C2
Unit cell:
a: 95.762Å b: 85.889Å c: 82.723Å
α: 90° β: 108.72° γ: 90°
R-values:
R R work R free
0.134 0.134 0.163
Expression system: Escherichia coli

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Citations

3 citation in other articles

Probing the enzymatic activity and maturation process of the EcAIII Ntn-amidohydrolase using local random mutagenesis.
Loch et al. (2024)
2 more

2 mentions without citation

Biochemical characterization of L-asparaginase isoforms from Rhizobium etli-the boosting effect of zinc.
Sliwiak et al. (2024)
1 more