8ott

Electron Microscopy
3.3Å resolution

Function and Biology Details

Cellular component:

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
PDBe Complex ID:
PDB-CPX-233834 (preferred)
Entry contents:
7 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (9 distinct):
Histone H3.1 Chains: A, E
Molecule details ›
Chains: A, E
Length: 95 amino acids
Theoretical weight: 11.18 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P68431 (Residues: 40-134; Coverage: 70%)
Gene names: H3C1, H3C10, H3C11, H3C12, H3C2, H3C3, H3C4, H3C6, H3C7, H3C8, H3FA, H3FB, H3FC HIST1H3C, H3FD, H3FF, H3FH, H3FI, H3FJ, H3FK, H3FL, HIST1H3A, HIST1H3B, HIST1H3D, HIST1H3E, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J
Sequence domains: Core histone H2A/H2B/H3/H4
Histone H4 Chains: B, F
Molecule details ›
Chains: B, F
Length: 82 amino acids
Theoretical weight: 9.28 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: P62805 (Residues: 22-103; Coverage: 80%)
Gene names: H4-16, H4/A, H4/B, H4/C, H4/D, H4/E, H4/G, H4/H, H4/I, H4/J, H4/K, H4/M, H4/N, H4/O, H4C1, H4C11, H4C12, H4C13, H4C14, H4C15, H4C16, H4C2, H4C3, H4C4, H4C5, H4C6, H4C8, H4C9, H4F2, H4FA, H4FB, H4FC, H4FD, H4FE, H4FG, H4FH, H4FI, H4FJ, H4FK, H4FM, H4FN, H4FO, HIST1H4A, HIST1H4B, HIST1H4C, HIST1H4D, HIST1H4E, HIST1H4F, HIST1H4H, HIST1H4I, HIST1H4J, HIST1H4K, HIST1H4L, HIST2H4, HIST2H4A, HIST2H4B, HIST4H4
Sequence domains: Centromere kinetochore component CENP-T histone fold
Histone H2A type 1-B/E Chain: C
Molecule details ›
Chain: C
Length: 109 amino acids
Theoretical weight: 11.9 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: P04908 (Residues: 9-117; Coverage: 84%)
Gene names: H2AC4, H2AC8, H2AFA, H2AFM, HIST1H2AB, HIST1H2AE
Sequence domains:
Histone H2B type 1-J Chains: D, H
Molecule details ›
Chains: D, H
Length: 93 amino acids
Theoretical weight: 10.33 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: P06899 (Residues: 33-125; Coverage: 74%)
Gene names: H2BC11, H2BFR, HIST1H2BJ
Sequence domains: Core histone H2A/H2B/H3/H4
Histone H2A type 1-B/E Chain: G
Molecule details ›
Chain: G
Length: 108 amino acids
Theoretical weight: 11.81 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: P04908 (Residues: 11-118; Coverage: 83%)
Gene names: H2AC4, H2AC8, H2AFA, H2AFM, HIST1H2AB, HIST1H2AE
Sequence domains:
Myc proto-oncogene protein Chain: M
Molecule details ›
Chain: M
Length: 53 amino acids
Theoretical weight: 6.35 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01106 (Residues: 368-420; Coverage: 12%)
Gene names: BHLHE39, MYC
Sequence domains: Helix-loop-helix DNA-binding domain
Protein max Chain: N
Molecule details ›
Chain: N
Length: 51 amino acids
Theoretical weight: 6.03 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P61244 (Residues: 23-73; Coverage: 32%)
Gene names: BHLHD4, MAX
Sequence domains: Helix-loop-helix DNA-binding domain
DNA (144-MER) Chain: I
Molecule details ›
Chain: I
Length: 144 nucleotides
Theoretical weight: 44.23 KDa
DNA (144-MER) Chain: J
Molecule details ›
Chain: J
Length: 144 nucleotides
Theoretical weight: 44.67 KDa

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.3Å
Relevant EMDB volumes: EMD-17184
Expression systems:
  • Escherichia coli
  • Escherichia coli 'BL21-Gold(DE3)pLysS AG'