› 8ow9

X-ray diffraction
2.7Å resolution

Crystal structure of Tannerella forsythia MurNAc kinase MurK in complex with N-acetylmuramic acid (MurNAc)

Released:
DOI: 10.2210/pdb8ow9/pdb
PDB entry 8ow9 coloured by chain, front view.
PDB entry 8ow9 coloured by chain, side view.
PDB entry 8ow9 coloured by chain, top view.
Source organism: Tannerella forsythia
Primary publication:
N-acetylmuramic acid recognition by MurK kinase from the MurNAc auxotrophic oral pathogen Tannerella forsythia.
Stasiak AC, Gogler K, Borisova M, Fink P, Mayer C, Stehle T, Zocher G
OpenAccess logo J Biol Chem 105076 (2023)
PMID: 37481208

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-222979 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ATPase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 292 amino acids
Theoretical weight: 32.48 KDa
Source organism: Tannerella forsythia
Expression system: Escherichia coli
UniProt:
  • Canonical: G8UQH9 (Residues: 2-280; Coverage: 99%)
Gene name: BFO_0042

Ligands and Environments

1 bound ligand:
Ligand AMU 4 x AMU
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: I4
Unit cell:
a: 113.425Å b: 113.425Å c: 294.762Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.269 0.257 0.276
Expression system: Escherichia coli

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Citations

1 mention without citation

N-acetylmuramic acid recognition by MurK kinase from the MurNAc auxotrophic oral pathogen Tannerella forsythia.
Stasiak et al. (2023)