8oz0

Electron Microscopy
3.5Å resolution

Structure of a human 48S translation initiation complex with eIF4F and eIF4A

Released:

Function and Biology Details

Reactions catalysed:
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
GTP + H(2)O = GDP + phosphate
The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero 57-mer (preferred)
PDBe Complex ID:
PDB-CPX-271447 (preferred)
Entry contents:
53 distinct polypeptide molecules
3 distinct RNA molecules
Macromolecules (56 distinct):
Eukaryotic translation initiation factor 3 subunit E Chain: 5
Molecule details ›
Chain: 5
Length: 445 amino acids
Theoretical weight: 52.28 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P60228 (Residues: 1-445; Coverage: 100%)
Gene names: EIF3E, EIF3S6, INT6
Sequence domains:
Eukaryotic translation initiation factor 3 subunit F Chain: 6
Molecule details ›
Chain: 6
Length: 357 amino acids
Theoretical weight: 37.59 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: O00303 (Residues: 1-357; Coverage: 100%)
Gene names: EIF3F, EIF3S5
Sequence domains:
Eukaryotic translation initiation factor 3 subunit G Chain: 7
Eukaryotic translation initiation factor 3 subunit H Chain: 8
Small ribosomal subunit protein eS32 Chain: 9
Eukaryotic translation initiation factor 3 subunit A Chain: A
Eukaryotic translation initiation factor 3 subunit K Chain: B
Eukaryotic translation initiation factor 3 subunit M Chain: C
Eukaryotic translation initiation factor 2 subunit 1 Chain: D
Eukaryotic translation initiation factor 2 subunit 3 Chain: E
Eukaryotic translation initiation factor 3 subunit I Chain: F
Eukaryotic translation initiation factor 1A, X-chromosomal Chain: G
Eukaryotic translation initiation factor 5 Chain: H
Eukaryotic translation initiation factor 3 subunit B Chain: I
Eukaryotic translation initiation factor 3 subunit C Chain: J
Eukaryotic translation initiation factor 3 subunit L Chain: K
Small ribosomal subunit protein eS7 Chain: L
Small ribosomal subunit protein eS27 Chain: M
Small ribosomal subunit protein eS21 Chain: N
Small ribosomal subunit protein uS5 Chain: O
Small ribosomal subunit protein eS1 Chain: P
Small ribosomal subunit protein uS2 Chain: Q
Small ribosomal subunit protein eS26 Chain: R
Small ribosomal subunit protein eS6 Chain: S
Small ribosomal subunit protein uS11 Chain: T
Eukaryotic translation initiation factor 2 subunit 2 Chain: U
Small ribosomal subunit protein uS15 Chain: V
Small ribosomal subunit protein uS17 Chain: X
Small ribosomal subunit protein eS4, X isoform Chain: Y
Small ribosomal subunit protein uS4 Chain: Z
Small ribosomal subunit protein uS12 Chain: a
Small ribosomal subunit protein eS30 Chain: b
Small ribosomal subunit protein uS8 Chain: c
Small ribosomal subunit protein eS8 Chain: d
Small ribosomal subunit protein eS24 Chain: e
Small ribosomal subunit protein uS7 Chain: f
Small ribosomal subunit protein uS9 Chain: g
Small ribosomal subunit protein uS3 Chain: h
Small ribosomal subunit protein eS10 Chain: i
Small ribosomal subunit protein uS19 Chain: j
Small ribosomal subunit protein RACK1 Chain: k
Small ribosomal subunit protein eS19 Chain: l
Small ribosomal subunit protein eS25 Chain: m
Small ribosomal subunit protein uS13 Chain: n
Small ribosomal subunit protein uS14 Chain: o
Ubiquitin-ribosomal protein eS31 fusion protein Chain: p
Small ribosomal subunit protein eS12 Chain: q
Small ribosomal subunit protein eS28 Chain: s
Small ribosomal subunit protein eS17 Chain: v
Small ribosomal subunit protein uS10 Chain: w
Eukaryotic translation initiation factor 3 subunit D Chain: x
Eukaryotic initiation factor 4A-I Chains: 1, 3
Eukaryotic translation initiation factor 4 gamma 1 Chain: 2
18S Ribosomal RNA Chain: W
tRNAiMet Chain: y
Messenger RNA Chain: z

Ligands and Environments

Experiments and Validation Details

Entry percentile scores
Resolution: 3.5Å
Relevant EMDB volumes: EMD-17297
Expression systems:
  • Homo sapiens
  • Escherichia coli BL21(DE3)
  • Baculovirus expression vector pFastBac1-HM