8p09

Electron Microscopy
3.3Å resolution

48S late-stage initiation complex with non methylated mRNA

Released:

Function and Biology Details

Reaction catalysed:
The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero 40-mer (preferred)
PDBe Complex ID:
PDB-CPX-275607 (preferred)
Entry contents:
37 distinct polypeptide molecules
3 distinct RNA molecules
Macromolecules (40 distinct):
Eukaryotic translation initiation factor 2 subunit 1 Chain: A
Molecule details ›
Chain: A
Length: 284 amino acids
Theoretical weight: 32.79 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1T2G4 (Residues: 1-284; Coverage: 100%)
Gene name: EIF2S1
Sequence domains:
Small ribosomal subunit protein uS2 Chain: C
Molecule details ›
Chain: C
Length: 207 amino acids
Theoretical weight: 23.27 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1TLT8 (Residues: 3-209; Coverage: 70%)
Gene name: RPSA
Sequence domains: Ribosomal protein S2
Small ribosomal subunit protein eS1 Chain: D
40S ribosomal protein S2 Chain: E
Small ribosomal subunit protein uS3 Chain: F
Small ribosomal subunit protein eS4 Chain: G
Small ribosomal subunit protein uS7 Chain: H
40S ribosomal protein S6 Chain: I
Small ribosomal subunit protein eS7 Chain: J
Small ribosomal subunit protein eS8 Chain: K
Small ribosomal subunit protein uS4 Chain: L
Small ribosomal subunit protein eS10 Chain: M
Small ribosomal subunit protein uS17 Chain: N
Small ribosomal subunit protein eS12 Chain: O
Small ribosomal subunit protein uS15 Chain: P
Small ribosomal subunit protein uS11 Chain: Q
Small ribosomal subunit protein uS19 Chain: R
Small ribosomal subunit protein uS9 Chain: S
Small ribosomal subunit protein eS17 Chain: T
Small ribosomal subunit protein uS13 Chain: U
Small ribosomal subunit protein eS19 Chain: V
Small ribosomal subunit protein uS10 Chain: W
Small ribosomal subunit protein eS21 Chain: X
Small ribosomal subunit protein uS8 Chain: Y
Small ribosomal subunit protein uS12 Chain: Z
Small ribosomal subunit protein eS24 Chain: a
Small ribosomal subunit protein eS26 Chain: b
Small ribosomal subunit protein eS27 Chain: c
Small ribosomal subunit protein eS28 Chain: d
Small ribosomal subunit protein uS14 Chain: e
Small ribosomal subunit protein eS31 Chain: f
Small ribosomal subunit protein RACK1 Chain: g
Ubiquitin-like FUBI-ribosomal protein eS30 fusion protein Chain: i
Eukaryotic translation initiation factor 4C Chain: j
ATP binding cassette subfamily E member 1 Chain: k
Small ribosomal subunit protein eS32 Chain: l
Small ribosomal subunit protein eS25 Chain: n
initiator methionylated tRNA Chain: 1
18S Ribosomal RNA Chain: 2
Messenger RNA Chain: 3

Ligands and Environments

1 bound ligand:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
Resolution: 3.3Å
Relevant EMDB volumes: EMD-17330