› 8p0d

X-ray diffraction
1.31Å resolution

Human 14-3-3 sigma in complex with human MDM2 peptide

Released:
DOI: 10.2210/pdb8p0d/pdb
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PDB entry 8p0d coloured by chain, front view.
PDB entry 8p0d coloured by chain, side view.
PDB entry 8p0d coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Characterizing the protein-protein interaction between MDM2 and 14-3-3σ; proof of concept for small molecule stabilization.
Ward JA, Romartinez-Alonso B, Kay DF, Bellamy-Carter J, Thurairajah B, Basran J, Kwon H, Leney AC, Macip S, Roversi P, Muskett FW, Doveston RG
J Biol Chem 300 105651 (2024)
PMID: 38237679

Function and Biology Details

Reaction catalysed: 
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152151 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
14-3-3 protein sigma Chain: A
Molecule details ›
Chain: A
Length: 259 amino acids
Theoretical weight: 29.55 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P31947 (Residues: 1-231; Coverage: 93%)
Gene names: HME1, SFN
Sequence domains: 14-3-3 protein
E3 ubiquitin-protein ligase Mdm2 Chain: B
Molecule details ›
Chain: B
Length: 31 amino acids
Theoretical weight: 3.84 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q00987 (Residues: 161-191; Coverage: 6%)
Gene name: MDM2

Ligands and Environments

4 bound ligands:
Ligand GOL 1 x GOL
Ligand PEG 1 x PEG
Ligand CL 1 x CL
Ligand MG 2 x MG
2 modified residues:
Ligand CSD 1 x CSD
Ligand SEP 2 x SEP

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: C2221
Unit cell:
a: 82.588Å b: 111.824Å c: 62.551Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.179 0.178 0.204
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

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Citations

1 citations in other articles

Rapid flow-based synthesis of post-translationally modified peptides and proteins: a case study on MYC's transactivation domain.
Williams et al. (2024)

1 mention without citation

Characterizing the protein-protein interaction between MDM2 and 14-3-3σ; proof of concept for small molecule stabilization.
Ward et al. (2024)

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