› 8p0i

X-ray diffraction
3.5Å resolution

Crystal structure of the open conformation of insulin-regulated aminopeptidase in complex with a small-MW inhibitor

Released:
DOI: 10.2210/pdb8p0i/pdb
PDB entry 8p0i coloured by chain, front view.
PDB entry 8p0i coloured by chain, side view.
PDB entry 8p0i coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Stabilization of the open conformation οf insulin-regulated aminopeptidase by a novel substrate-selective small-molecule inhibitor.
Mpakali A, Georgaki G, Buson A, Findlay AD, Foot JS, Mauvais FX, van Endert P, Giastas P, Hamprecht DW, Stratikos E
Protein Sci 33 e5151 (2024)
PMID: 39167040

Function and Biology Details

Reaction catalysed: 
Release of an N-terminal amino acid, Cys-|-Xaa-, in which the half-cystine residue is involved in a disulfide loop, notably in oxytocin or vasopressin. Hydrolysis rates on a range of aminoacyl arylamides exceed that for the cystinyl derivative, however.
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193794 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (4 distinct):
Leucyl-cystinyl aminopeptidase, pregnancy serum form Chains: A, B
Molecule details ›
Chains: A, B
Length: 871 amino acids
Theoretical weight: 100.14 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q9UIQ6 (Residues: 155-1025; Coverage: 85%)
Gene names: LNPEP, OTASE
Sequence domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
3 bound ligands:
Ligand NAG 13 x NAG
Ligand WC3 2 x WC3
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Spacegroup: P21
Unit cell:
a: 68.229Å b: 255.121Å c: 73.274Å
α: 90° β: 110° γ: 90°
R-values:
R R work R free
0.24 0.24 0.277
Expression system: Homo sapiens

Quick links

Citations

1 mention without citation

Stabilization of the open conformation οf insulin-regulated aminopeptidase by a novel substrate-selective small-molecule inhibitor.
Mpakali et al. (2024)