› 8p19

X-ray diffraction
2.45Å resolution

USP28 USP domain apo

Released:
DOI: 10.2210/pdb8p19/pdb
PDB entry 8p19 coloured by chain, front view.
PDB entry 8p19 coloured by chain, side view.
PDB entry 8p19 coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Sauer F, Karal Nair R, Kisker C

Function and Biology Details

Reaction catalysed: 
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188742 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ubiquitin carboxyl-terminal hydrolase 28 Chain: A
Molecule details ›
Chain: A
Length: 377 amino acids
Theoretical weight: 44.03 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q96RU2 (Residues: 149-399, 580-698; Coverage: 34%)
  • Best match: Q96RU2-3 (Residues: 149-399, 406-406)
Gene names: KIAA1515, USP28
Sequence domains: Ubiquitin carboxyl-terminal hydrolase

Ligands and Environments

2 bound ligands:
Ligand EDO 1 x EDO
Ligand CL 4 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: R32
Unit cell:
a: 106.634Å b: 106.634Å c: 329.857Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.195 0.194 0.222
Expression system: Escherichia coli

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