› 8p1q

X-ray diffraction
2.79Å resolution

USP28 in complex with FT206

Released:
DOI: 10.2210/pdb8p1q/pdb
PDB entry 8p1q coloured by chain, front view.
PDB entry 8p1q coloured by chain, side view.
PDB entry 8p1q coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Sauer F, Karal Nair R, Kisker C

Function and Biology Details

Reaction catalysed: 
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188743 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ubiquitin carboxyl-terminal hydrolase 28 Chains: A, B
Molecule details ›
Chains: A, B
Length: 494 amino acids
Theoretical weight: 57.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q96RU2 (Residues: 149-458, 529-707; Coverage: 45%)
Gene names: KIAA1515, USP28
Sequence domains: Ubiquitin carboxyl-terminal hydrolase

Ligands and Environments

2 bound ligands:
Ligand DMS 2 x DMS
Ligand WFT 2 x WFT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Unit cell:
a: 100.444Å b: 105.541Å c: 178.708Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.213 0.212 0.236
Expression system: Escherichia coli

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