› 8p1u

Electron Microscopy
3.3Å resolution

Structure of divisome complex FtsWIQLB

Released:
DOI: 10.2210/pdb8p1u/pdb
PDB entry 8p1u coloured by chain, front view.
PDB entry 8p1u coloured by chain, side view.
PDB entry 8p1u coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
Structural insights into the activation of the divisome complex FtsWIQLB.
Yang L, Chen Y, Chang S, Shen C, Wang X, Zhang C, Zhang Z, Ding BS, Su Z, Dong H, Tang X
OpenAccess logo Cell Discov 10 2 (2024)
PMID: 38172099
Related structures: EMD-17356

Function and Biology Details

Reactions catalysed: 
(GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala))(n)-diphosphoundecaprenol + GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol = (GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala))(n+1)-diphosphoundecaprenol + undecaprenyl diphosphate
Preferential cleavage: (Ac)(2)-L-Lys-D-Ala-|-D-Ala. Also transpeptidation of peptidyl-alanyl moieties that are N-acyl substituents of D-alanine.
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-216214 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Cell division protein FtsL Chain: C
Molecule details ›
Chain: C
Length: 97 amino acids
Theoretical weight: 11.15 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: Q9HVZ6 (Residues: 1-97; Coverage: 100%)
Gene names: PA4419, ftsL
Sequence domains: Cell division protein FtsL
Probable peptidoglycan glycosyltransferase FtsW Chain: A
Molecule details ›
Chain: A
Length: 399 amino acids
Theoretical weight: 43.79 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: Q9HW00 (Residues: 1-399; Coverage: 100%)
Gene names: PA4413, ftsW
Sequence domains: Cell cycle protein
Peptidoglycan D,D-transpeptidase FtsI Chain: B
Molecule details ›
Chain: B
Length: 579 amino acids
Theoretical weight: 62.93 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: G3XD46 (Residues: 1-579; Coverage: 100%)
Gene names: PA4418, ftsI, pbpB
Sequence domains:
Cell division protein FtsB Chain: D
Molecule details ›
Chain: D
Length: 94 amino acids
Theoretical weight: 10.89 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: Q9HXZ6 (Residues: 1-94; Coverage: 100%)
Gene names: PA3634, ftsB
Sequence domains: Septum formation initiator
Cell division protein FtsQ Chain: E
Molecule details ›
Chain: E
Length: 287 amino acids
Theoretical weight: 32.29 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: G3XDA7 (Residues: 1-287; Coverage: 100%)
Gene names: PA4409, ftsQ
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.3Å
Relevant EMDB volumes: EMD-17356
Expression system: Escherichia coli K-12

Quick links