› 8p23

Electron Microscopy
3.17Å resolution

Cryo-EM structure of the anaerobic ribonucleotide reductase from Prevotella copri in its dimeric, ATP/CTP-bound state

Released:
DOI: 10.2210/pdb8p23/pdb
PDB entry 8p23 coloured by chain, front view.
PDB entry 8p23 coloured by chain, side view.
PDB entry 8p23 coloured by chain, top view.
Source organism: Prevotella copri
Primary publication:
Activity modulation in anaerobic ribonucleotide reductase: nucleotide binding to the ATP-cone mediates long-range order-disorder transitions in the active site
Bimai O, Banerjee I, Rozman Grinberg I, Huang P, Lundin D, Sjoberg BM, Logan DT
Elife (2023)
Related structures: EMD-17357

Function and Biology Details

Reaction catalysed: 
2'-deoxyribonucleoside triphosphate + thioredoxin disulfide + H(2)O = ribonucleoside triphosphate + thioredoxin
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-239830 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ATP-cone domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 743 amino acids
Theoretical weight: 84.64 KDa
Source organism: Prevotella copri
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A3E4SF67 (Residues: 1-739; Coverage: 100%)
Gene names: DW250_07000, DW262_07430, DW263_04390, DXC61_11830
Sequence domains:

Ligands and Environments

3 bound ligands:
Ligand ATP 4 x ATP
Ligand CTP 1 x CTP
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.17Å
Relevant EMDB volumes: EMD-17357
Expression system: Escherichia coli

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