8p4r

Electron Microscopy
11.9Å resolution

In situ structure average of GroEL14-GroES14 complexes in Escherichia coli cytosol obtained by cryo electron tomography

Released:

Function and Biology Details

Reaction catalysed:
ATP + H(2)O + a folded polypeptide = ADP + phosphate + an unfolded polypeptide
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero 28-mer (preferred)
PDBe Complex ID:
PDB-CPX-268791 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Chaperonin GroEL Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N
Length: 547 amino acids
Theoretical weight: 57.26 KDa
Source organism: Escherichia coli BL21(DE3)
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A140NH65 (Residues: 2-548; Coverage: 100%)
Gene names: ECBD_3888, groEL, groL
Sequence domains: TCP-1/cpn60 chaperonin family
Co-chaperonin GroES Chains: O, P, Q, R, S, T, U, V, W, X, Y, Z, a, b
Molecule details ›
Chains: O, P, Q, R, S, T, U, V, W, X, Y, Z, a, b
Length: 97 amino acids
Theoretical weight: 10.4 KDa
Source organism: Escherichia coli BL21(DE3)
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A140NEN6 (Residues: 1-97; Coverage: 100%)
Gene names: ECBD_3889, groES, groS
Sequence domains: Chaperonin 10 Kd subunit

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 11.9Å
Relevant EMDB volumes: EMD-17426
Expression system: Escherichia coli BL21(DE3)