› 8p53

Electron Microscopy
2.7Å resolution

Cryo-EM structure of the c-di-GMP-free FleQ-FleN master regulator complex of P. aeruginosa

Released:
DOI: 10.2210/pdb8p53/pdb
PDB entry 8p53 coloured by chain, front view.
PDB entry 8p53 coloured by chain, side view.
PDB entry 8p53 coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Primary publication:
Structures of the P. aeruginosa FleQ-FleN master regulators reveal large-scale conformational switching in motility and biofilm control.
Torres-Sánchez L, Géry Sana T, Decossas M, Hashem Y, Krasteva PV
OpenAccess logo Proc Natl Acad Sci U S A 120 e2312276120 (2023)
PMID: 38051770
Related structures: EMD-17445

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-236467 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Antiactivator FleN Chains: A, B
Molecule details ›
Chains: A, B
Length: 307 amino acids
Theoretical weight: 33.24 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: G3XD64 (Residues: 2-280; Coverage: 100%)
Gene names: PA1454, fleN
Sequence domains: NUBPL iron-transfer P-loop NTPase
Transcriptional regulator FleQ Chains: C, D, E, F
Molecule details ›
Chains: C, D, E, F
Length: 492 amino acids
Theoretical weight: 55.49 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: G3XCV0 (Residues: 2-490; Coverage: 100%)
Gene names: PA1097, fleQ
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand MG 2 x MG
Ligand ACP 2 x ACP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.7Å
Relevant EMDB volumes: EMD-17445
Expression system: Escherichia coli BL21(DE3)

Quick links