› 8p5y

X-ray diffraction
1.88Å resolution

Artificial transfer hydrogenase with a Mn-12 cofactor and Streptavidin S112Y-K121M mutant

Released:
DOI: 10.2210/pdb8p5y/pdb
Model geometry
Fit model/data
PDB entry 8p5y coloured by chain, front view.
PDB entry 8p5y coloured by chain, side view.
PDB entry 8p5y coloured by chain, top view.
Source organism: Streptomyces avidinii
Primary publication:
Manganese Transfer Hydrogenases Based on the Biotin-Streptavidin Technology.
Wang W, Tachibana R, Zou Z, Chen D, Zhang X, Lau K, Pojer F, Ward T, Hu X
Angew Chem Int Ed Engl e202311896 (2023)
PMID: 37671593

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149694 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Streptavidin Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 159 amino acids
Theoretical weight: 16.65 KDa
Source organism: Streptomyces avidinii
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P22629 (Residues: 39-183; Coverage: 91%)
Sequence domains: Avidin family

Ligands and Environments

4 bound ligands:
Ligand WZQ 4 x WZQ
Ligand MN 4 x MN
Ligand BR 4 x BR
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SLS BEAMLINE X06DA
Spacegroup: C2
Unit cell:
a: 103.437Å b: 51.198Å c: 92.535Å
α: 90° β: 117.457° γ: 90°
R-values:
R R work R free
0.199 0.196 0.265
Expression system: Escherichia coli BL21(DE3)

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