› 8p6i

X-ray diffraction
2.5Å resolution

Crystal structure of the 139H2 Fab fragment bound to Muc1 peptide epitope

Released:
DOI: 10.2210/pdb8p6i/pdb
PDB entry 8p6i coloured by chain, front view.
PDB entry 8p6i coloured by chain, side view.
PDB entry 8p6i coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Reverse-engineering the anti-MUC1 antibody 139H2 by mass spectrometry-based de novo sequencing.
Peng W, Giesbers KC, Šiborová M, Beugelink JW, Pronker MF, Schulte D, Hilkens J, Janssen BJ, Strijbis K, Snijder J
OpenAccess logo Life Sci Alliance 7 (2024)
PMID: 38508723

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-242590 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Mucin-1 subunit alpha Chains: A, a
Molecule details ›
Chains: A, a
Length: 9 amino acids
Theoretical weight: 882 Da
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P15941 (Residues: 921-929; Coverage: 1%)
Gene names: MUC1, PUM
139H2 HC Chains: H, h
Molecule details ›
Chains: H, h
Length: 236 amino acids
Theoretical weight: 25.49 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
139H2 LC Chains: L, l
Molecule details ›
Chains: L, l
Length: 220 amino acids
Theoretical weight: 24.44 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: C2
Unit cell:
a: 211.833Å b: 42.757Å c: 129.064Å
α: 90° β: 122.306° γ: 90°
R-values:
R R work R free
0.206 0.203 0.254
Expression systems:
  • Not provided
  • Homo sapiens

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