› 8p8a

Electron Microscopy
3.2Å resolution

Structure of 5D3-Fab and nanobody(Nb17)-bound ABCG2

Released:
DOI: 10.2210/pdb8p8a/pdb
PDB entry 8p8a coloured by chain, front view.
PDB entry 8p8a coloured by chain, side view.
PDB entry 8p8a coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis of the allosteric inhibition of human ABCG2 by nanobodies.
Irobalieva RN, Manolaridis I, Jackson SM, Ni D, Pardon E, Stahlberg H, Steyaert J, Locher KP
J Mol Biol 168234 (2023)
PMID: 37597690
Related structures: EMD-17543

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O + xenobiotic(Side 1) = ADP + phosphate + xenobiotic(Side 2)
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero heptamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-278855 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (5 distinct):
5D3(Fab) light chain variable domain Chains: C, E
Molecule details ›
Chains: C, E
Length: 214 amino acids
Theoretical weight: 23.59 KDa
Source organism: Mus musculus
Expression system: Mus musculus
5D3(Fab) heavy chain variable domain Chains: D, F
Molecule details ›
Chains: D, F
Length: 160 amino acids
Theoretical weight: 17.51 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Nanobody Chain: Z
Molecule details ›
Chain: Z
Length: 123 amino acids
Theoretical weight: 13.38 KDa
Source organism: Lama glama
Expression system: Escherichia coli
Broad substrate specificity ATP-binding cassette transporter ABCG2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 655 amino acids
Theoretical weight: 72.39 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.2Å
Relevant EMDB volumes: EMD-17543
Expression systems:
  • Mus musculus
  • Escherichia coli
  • Homo sapiens

Quick links

Citations

1 mention without citation

Structural View of Cryo-Electron Microscopy-Determined ATP-Binding Cassette Transporters in Human Multidrug Resistance.
Fan et al. (2024)