› 8p9c

X-ray diffraction
1.76Å resolution

Crystal structure of p63-p73 heterotetramer (tetramerisation domain) in complex with darpin 1810 F11

Released:
DOI: 10.2210/pdb8p9c/pdb
PDB entry 8p9c coloured by chain, front view.
PDB entry 8p9c coloured by chain, side view.
PDB entry 8p9c coloured by chain, top view.
Source organisms:
Primary publication:
DARPins detect the formation of hetero-tetramers of p63 and p73 in epithelial tissues and in squamous cell carcinoma.
Strubel A, Münick P, Hartmann O, Chaikuad A, Dreier B, Schaefer JV, Gebel J, Osterburg C, Tuppi M, Schäfer B, Buck V, Rosenfeldt M, Knapp S, Plückthun A, Diefenbacher ME, Dötsch V
OpenAccess logo Cell Death Dis 14 674 (2023)
PMID: 37828008

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-518516 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Tumor protein 63 Chain: A
Molecule details ›
Chain: A
Length: 61 amino acids
Theoretical weight: 7.38 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9H3D4 (Residues: 397-455; Coverage: 9%)
Gene names: KET, P63, P73H, P73L, TP63, TP73L
Sequence domains: P53 tetramerisation motif
Tumor protein p73 Chain: B
Molecule details ›
Chain: B
Length: 50 amino acids
Theoretical weight: 6.03 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O15350 (Residues: 351-398; Coverage: 8%)
Gene names: P73, TP73
Sequence domains: P53 tetramerisation motif
Darpin 1810 F11 Chain: C
Molecule details ›
Chain: C
Length: 159 amino acids
Theoretical weight: 16.87 KDa
Source organism: Lama glama
Expression system: Escherichia coli BL21(DE3)

Ligands and Environments

1 bound ligand:
Ligand EDO 4 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: C2
Unit cell:
a: 84.024Å b: 60.312Å c: 53.576Å
α: 90° β: 100.26° γ: 90°
R-values:
R R work R free
0.172 0.17 0.21
Expression systems:
  • Escherichia coli
  • Escherichia coli BL21(DE3)

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