› 8pa3

X-ray diffraction
1.07Å resolution

Crystal structure of the metallo-beta-lactamase VIM1 with 2500

Released:
DOI: 10.2210/pdb8pa3/pdb
PDB entry 8pa3 coloured by chain, front view.
PDB entry 8pa3 coloured by chain, side view.
PDB entry 8pa3 coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Entry authors: Calvopina K, Brem J, Farley AJM, Allen MD, Schofield CJ

Function and Biology Details

Reaction catalysed: 
A beta-lactam + H(2)O = a substituted beta-amino acid
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194976 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Metallo-beta-lactamase type 2 Chain: A
Molecule details ›
Chain: A
Length: 266 amino acids
Theoretical weight: 28.05 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9XAY4 (Residues: 1-266; Coverage: 100%)
Gene names: CAZ10_38240, CAZ10_38245, blaVIM, blaVIM-1
Sequence domains: Metallo-beta-lactamase superfamily

Ligands and Environments

2 bound ligands:
Ligand ZN 2 x ZN
Ligand XNT 1 x XNT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P21
Unit cell:
a: 39.51Å b: 68.03Å c: 40.4Å
α: 90° β: 93.74° γ: 90°
R-values:
R R work R free
0.125 0.124 0.143
Expression system: Escherichia coli BL21(DE3)

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