› 8pcc

X-ray diffraction
2.04Å resolution

Structure of serine-beta-lactamase CTX-M-14 following the time-resolved active site binding of boric acid, 100 ms

Released:
DOI: 10.2210/pdb8pcc/pdb
PDB entry 8pcc coloured by chain, front view.
PDB entry 8pcc coloured by chain, side view.
PDB entry 8pcc coloured by chain, top view.
Source organism: Klebsiella pneumoniae
Primary publication:
Time-resolved crystallography of boric acid binding to the active site serine of the β-lactamase CTX-M-14 and subsequent 1,2-diol esterification.
Prester A, Perbandt M, Galchenkova M, Oberthuer D, Werner N, Henkel A, Maracke J, Yefanov O, Hakanpää J, Pompidor G, Meyer J, Chapman H, Aepfelbacher M, Hinrichs W, Rohde H, Betzel C
OpenAccess logo Commun Chem 7 152 (2024)
PMID: 38969718

Function and Biology Details

Reaction catalysed: 
A beta-lactam + H(2)O = a substituted beta-amino acid
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-101890 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-lactamase Chain: A
Molecule details ›
Chain: A
Length: 263 amino acids
Theoretical weight: 28 KDa
Source organism: Klebsiella pneumoniae
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0H3H219 (Residues: 29-291; Coverage: 100%)
Gene name: KPHS_p301310
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand BO4 1 x BO4
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, DESY BEAMLINE P11
Spacegroup: P3221
Unit cell:
a: 41.84Å b: 41.84Å c: 233.28Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.191 0.188 0.231
Expression system: Escherichia coli

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