› 8pda

Electron Microscopy
3.58Å resolution

cryo-EM structure of Doa10 with RING domain in MSP1E3D1

Released:
DOI: 10.2210/pdb8pda/pdb
PDB entry 8pda coloured by chain, front view.
PDB entry 8pda coloured by chain, side view.
PDB entry 8pda coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Doa10/MARCH6 architecture interconnects E3 ligase activity with lipid-binding transmembrane channel to regulate SQLE.
Botsch JJ, Junker R, Sorgenfrei M, Ogger PP, Stier L, von Gronau S, Murray PJ, Seeger MA, Schulman BA, Bräuning B
OpenAccess logo Nat Commun 15 410 (2024)
PMID: 38195637
Related structures: EMD-17608

Function and Biology Details

Reaction catalysed: 
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154073 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ERAD-associated E3 ubiquitin-protein ligase DOA10 Chain: A
Molecule details ›
Chain: A
Length: 1319 amino acids
Theoretical weight: 151.61 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P40318 (Residues: 1-1319; Coverage: 100%)
Gene names: DOA10, SSM4, YI3299.01C, YI9905.18C, YIL030C
Sequence domains: RING-variant domain

Ligands and Environments

2 bound ligands:
Ligand PCW 3 x PCW
Ligand PX6 1 x PX6
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.58Å
Relevant EMDB volumes: EMD-17608
Expression system: Trichoplusia ni

Quick links