› 8pdk

X-ray diffraction
2Å resolution

X-ray structure of the Thermus thermophilus PilF-GSPIIB domain in the c-di-GMP bound state

Released:
DOI: 10.2210/pdb8pdk/pdb
PDB entry 8pdk coloured by chain, front view.
PDB entry 8pdk coloured by chain, side view.
PDB entry 8pdk coloured by chain, top view.
Source organism: Thermus thermophilus HB27
Entry authors: Neissner K, Woehnert J

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-274798 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Type IV pilus assembly ATPase PilB Chains: A, B
Molecule details ›
Chains: A, B
Length: 146 amino acids
Theoretical weight: 16.27 KDa
Source organism: Thermus thermophilus HB27
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5SLC9 (Residues: 159-302; Coverage: 16%)
Gene names: TTHA0364, pilB
Sequence domains: MshEN domain

Ligands and Environments

3 bound ligands:
Ligand C2E 2 x C2E
Ligand SO4 7 x SO4
Ligand ACT 13 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X6A
Spacegroup: R3
Unit cell:
a: 108.138Å b: 108.138Å c: 87.922Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.179 0.178 0.212
Expression system: Escherichia coli BL21(DE3)

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